Treatment of human sperm with serine protease during density gradient centrifugation
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چکیده
منابع مشابه
Comparison of Sperm Telomere Length between Two Sperm Selection Procedures: Density Gradient Centrifugation and Zeta Potential
Background: Telomeres are particular sequences of DNA located at the end of the eukaryotic chromosomes that are essential for genome integrity. Telomere length in spermatozoa differs among males, as well as spermatozoa. Also, decreased telomere length in spermatozoa of infertile men is associated with the reduction of fertility potential and embryo quality. Density gradient centrifugation (DGC)...
متن کاملcan zeta sperm selection method, recover sperm with higher dna integrity compare to density gradient centrifugation?
background: sperm selection for icsi based on morphology and motility might not be relevant to chromatin integrity. thus sperm selection based on sperm characteristics has been suggested. objective: the aim of this study was to compare the efficiency of zeta method with routine density gradient centrifugation method (dgc) for the selection of sperm with higher dna integrity. materials and metho...
متن کاملElimination of bacteria from human semen during sperm preparation using density gradient centrifugation with a novel tube insert.
The occurrence of bacteria in sperm samples intended for in vitro fertilisation can compromise the outcome of assisted reproductive techniques. Effective semen processing procedures should therefore be implemented to remove bacteria from semen. Unfortunately, technique failure does occur whereby bacteria can be found in processed sperm preparations. To improve the effectiveness of semen process...
متن کاملBull Fertility and Its Relation with Density Gradient Selected Sperm
Background sperm selection is generally used to describe methods for separation of spermatozoa for in-vitro-assisted reproduction. Few studies reported relation in vivo fertility and semen parameters after selection of fertile spermatozoa; hence, our research attempt to assess different semen parameters after post-thaw (PT) or after selecting sperm sample (SSp), using density gradient separatio...
متن کاملSperm quality after density gradient centrifugation with three commercially available media: a controlled trial
BACKGROUND Density gradient is the preferred technique for sperm processing for ART. However, no study has examined sperm quality using different processing media simultaneously and under identical conditions. Therefore, we evaluated semen quality following sperm preparation by three commonly used commercially available density gradient media in a well-designed controlled trial. METHODS We ob...
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ژورنال
عنوان ژورنال: Journal of Assisted Reproduction and Genetics
سال: 2012
ISSN: 1058-0468,1573-7330
DOI: 10.1007/s10815-012-9851-6